Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The AH-receptor: genetics, structure and function

H I Swanson1, C A Bradfield

  • 1Department of Pharmacology, Northwestern University Medical School, Chicago, IL 60611.

Pharmacogenetics
|October 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental Characterization of the Anatomical Structures of the Lumbar Spine Under Dynamic Sagittal Bending.

Biomedical sciences instrumentation·2015
Same author

Material Parameter Determination of an L4-L5 Motion Segment Finite Element Model Under High Loading Rates.

Biomedical sciences instrumentation·2015
Same author

Toxicity and recovery in the pregnant mouse after gestational exposure to the cyanobacterial toxin, cylindrospermopsin.

Journal of applied toxicology : JAT·2010
Same author

Activation of the aryl hydrocarbon receptor by TCDD inhibits senescence: a tumor promoting event?

Biochemical pharmacology·2008
Same author

Identification of kaempferol as an inhibitor of cigarette smoke-induced activation of the aryl hydrocarbon receptor and cell transformation.

Carcinogenesis·2006
Same author

Generation of allelic series in mice by gene targeting.

Methods in molecular biology (Clifton, N.J.)·2003
Same journal

No evidence of a role for PPARgamma Pro12Ala polymorphism in endometrial cancer susceptibility.

Pharmacogenetics·2004
Same journal

Pharmacogenetics and herb-drug interactions: experience with Ginkgo biloba and omeprazole.

Pharmacogenetics·2004
Same journal

Functional comparison of the endothelial nitric oxide synthase Glu298Asp polymorphic variants in human endothelial cells.

Pharmacogenetics·2004
Same journal

Response to micronized fenofibrate treatment is associated with the peroxisome-proliferator-activated receptors alpha G/C intron7 polymorphism in subjects with type 2 diabetes.

Pharmacogenetics·2004
Same journal

Upstream and coding region CYP2C9 polymorphisms: correlation with warfarin dose and metabolism.

Pharmacogenetics·2004
Same journal

The human serotonin receptor 2B: coding region polymorphisms and association with vulnerability to illegal drug abuse.

Pharmacogenetics·2004
See all related articles

The aryl hydrocarbon receptor (AH receptor) is a transcription factor regulating responses to environmental chemicals. Its structure, including PAS and bHLH domains, suggests a heterodimeric partnership for gene activation.

Area of Science:

  • Molecular Biology
  • Toxicology
  • Biochemistry

Background:

  • The aryl hydrocarbon receptor (AH receptor) is a ligand-activated transcription factor.
  • It plays a crucial role in biological responses to planar aromatic hydrocarbons and environmental toxins.
  • Its unique mechanism of gene activation and murine genetics have driven significant research interest.

Purpose of the Study:

  • To review recent experimental findings on the AH receptor.
  • To elucidate the molecular mechanisms of AH receptor-mediated signal transduction.
  • To develop a molecular model for AH receptor function.

Main Methods:

  • Cloning experiments revealing structural relationships to Per, ARNT, and Sim proteins.
  • Analysis of conserved domains: PAS domain for agonist binding and bHLH domain for DNA binding and heterodimerization.

Related Experiment Videos

  • Comparative analysis with known transcription factor partners like Myc/Max and MyoD/E2A.

    • Main Results:

    • The AH receptor shares structural homology with the Per, ARNT, and Sim (PAS) family.
    • The PAS domain contains a hydrophobic pocket for ligand binding.
    • The basic/helix-loop-helix (bHLH) domain facilitates heterodimerization and DNA binding.

    Conclusions:

    • The AH receptor and ARNT protein likely form heterodimeric partners.
    • This partnership activates gene expression through sequence-specific DNA binding.
    • A molecular model of AH receptor signal transduction is proposed based on these findings.