Separation of estrogens and rodenticides using capillary electrophoresis with aqueous-methanolic buffers

Capillary electrophoresis (CE) has proven to be an efficient method for the separation of various charged and neutral analytes. For analytes having limited solubility in water, the CE mode of separation has been micellar electrokinetic capillary chromatography (MECC). However, another approach is the direct addition of an organic solvent to a non-MECC CE separation system. Walbroehl and Jorgenson and also Balchunas and Sepaniak have reported on the use of organics in CE but the focus of their work was using MECC to separate small organic compounds. This work examines the use of aqueous-methanolic buffers in non-MECC CE separations of estrogens and rodenticides.