Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interleukin-11 inhibits bone formation in vitro

F J Hughes1, G L Howells

  • 1Department of Oral Medicine and Periodontology, London Hospital Medical College, UK.

Calcified Tissue International
|November 1, 1993
PubMed
Summary

Interleukin-11 (IL-11) inhibits bone formation by preventing osteoblast precursor differentiation. This cytokine is a potent inhibitor, impacting alkaline phosphatase expression and nodule formation in bone cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Periodontal diagnosis in the context of the 2017 classification system of periodontal diseases and conditions: presentation of a patient with periodontitis localised to the molar teeth.

British dental journal·2019
Same author

Periodontal diagnosis in the context of the 2017 classification system of periodontal diseases and conditions: Presentation of a middle-aged patient with localised periodontitis.

British dental journal·2019
Same author

Periodontal diagnosis in the context of the 2017 classification system of periodontal diseases and conditions - implementation in clinical practice.

British dental journal·2019
Same author

Periodontal diagnosis in the context of the BSP implementation plan for the 2017 classification system of periodontal diseases and conditions: presentation of a pair of young siblings with periodontitis.

British dental journal·2019
Same author

Comparative analysis of immune cell subsets in peripheral blood from patients with periodontal disease and healthy controls.

Clinical and experimental immunology·2018
Same author

Drugs, medications and periodontal disease.

British dental journal·2014

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Biology

Background:

  • Osteoblast precursors are crucial for bone formation.
  • Interleukin-11 (IL-11) is a cytokine with known roles in various cellular processes.
  • The specific impact of IL-11 on osteoblast differentiation requires further elucidation.

Purpose of the Study:

  • To investigate the effects of Interleukin-11 (IL-11) on the differentiation of osteoblast precursors.
  • To determine the potency of IL-11 in inhibiting bone nodule formation.
  • To compare the effects of IL-11 with Interleukin-6 (IL-6) on osteoblasts.

Main Methods:

  • Utilized a bone nodule forming assay in rat calvaria cell cultures.
  • Administered varying doses of IL-11 to assess dose-dependent effects.
  • Measured alkaline phosphatase expression as a marker of osteoblast differentiation.

Main Results:

  • IL-11 demonstrated a dose-dependent inhibition of bone nodule formation.
  • Complete inhibition of nodule formation was observed at 500 U/ml IL-11.
  • IL-11 significantly reduced alkaline phosphatase expression in the cultured cells.

Conclusions:

  • IL-11 acts as an osteotropic cytokine, influencing bone metabolism.
  • IL-11 exhibits more potent inhibitory effects on bone formation than IL-6.
  • IL-11 may play a significant role in regulating bone formation in both physiological and pathological conditions.

Related Experiment Videos