Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transient outward current in human and rat ventricular myocytes

E Wettwer1, G Amos, J Gath

  • 1Pharmakologisches Institut, University of Essen, Germany.

Cardiovascular Research
|September 1, 1993
PubMed
Summary

Transient outward currents (I(to)) in human ventricular myocytes from failing and non-failing hearts showed no significant differences. These human cardiac currents share similarities with rat myocytes, suggesting I(to) as a potential antiarrhythmic drug target.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An app with brief behavioural support to promote physical activity after a cancer diagnosis (APPROACH): study protocol for a pilot randomised controlled trial.

Pilot and feasibility studies·2022
Same author

Bridging the age gap in breast cancer: impact of omission of breast cancer surgery in older women with oestrogen receptor-positive early breast cancer on quality-of-life outcomes.

The British journal of surgery·2021
Same author

Bridging the age gap in breast cancer: cluster randomized trial of two decision support interventions for older women with operable breast cancer on quality of life, survival, decision quality, and treatment choices.

The British journal of surgery·2021
Same author

Cardiac heterogeneity and drug-provoked arrhythmias.

Acta physiologica (Oxford, England)·2017
Same author

Observational study of the development and evaluation of a fertility preservation patient decision aid for teenage and adult women diagnosed with cancer: the Cancer, Fertility and Me research protocol.

BMJ open·2017
Same author

[ESC guidelines on atrial fibrillation 2016 : Summary of the most relevant recommendations and modifications].

Herz·2016

Area of Science:

  • Cardiovascular Physiology
  • Cardiac Electrophysiology

Background:

  • Transient outward currents (I(to)) play a crucial role in cardiac action potential repolarization.
  • Understanding I(to) in human hearts is essential for developing targeted therapies for cardiac arrhythmias.

Purpose of the Study:

  • To investigate and compare the electrophysiological characteristics of transient outward currents (I(to)) in human ventricular myocytes from failing and non-failing hearts.
  • To compare human I(to) with that of rat myocytes.

Main Methods:

  • Whole-cell patch clamp technique was employed to study depolarisation-dependent I(to) in isolated human ventricular myocytes.
  • Human myocytes were obtained from heart transplant recipients (failing hearts) and multiorgan donors (non-failing hearts).
  • Electrophysiological properties were compared between human and rat myocytes.

Related Experiment Videos

Main Results:

  • I(to) was present in ventricular myocytes from both failing and non-failing human hearts, with similar current densities.
  • Human I(to) exhibited slower activation and faster inactivation kinetics compared to rat I(to).
  • The late component of outward current in human cells showed no potential-dependent inactivation, unlike in rat cells.

Conclusions:

  • Transient outward currents (I(to)) in human ventricular myocytes do not show dramatic differences between failing and non-failing hearts.
  • Human I(to) characteristics are similar, though not identical, to those observed in rat heart cells.
  • I(to) represents a potential therapeutic target for antiarrhythmic drug development.