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Cell-cycle-stage specificity of the methotrexate block as resolved by X-ray-induced chromosome damage
V B Richardson1, L G Littlefield, A M Sayer
1Department of Biology, Tuskegee University, AL 36088.
Cytogenetics and Cell Genetics
|January 1, 1994
Abstract:
Radiation-induced chromosome aberrations were used as biomarkers to compare G0, mid-G1, and methotrexate (MTX)-arrested lymphocytes. The ratio of chromosome-type to chromatid-type aberrations in MTX-arrested cells was consistent with that predicted when postreplicative chromosomes are exposed to ionizing radiation and supports the premise that MTX arrests cells in late S/G2 of the cell cycle.