Vascular contractile properties in hypertension. I. A study of testing methods

Abnormal contractile responses of blood vessels are observed widely in several hypertension models and are assumed to play a role in the etiology of essential hypertension. Inconsistent reports also exist due to different animals, preparations, and experimental techniques employed. In this investigation, we compared vascular response of normotensive Wistar-Kyoto rat (WKY) with that of spontaneously hypertensive rat (SHR) employing two experimental methods. Thoracic aorta obtained from age-matched animals were contracted by norepinephrine (NE) with either--single-dose method or cumulative-dose method. Differences in both reactivity (maximal tension) and sensitivity (ED50) to NE were observed. Although no significant differences were found between WKY and SHR with either method, the choice of the method employed could affect the outcome. For aorta from WKY, the reactivity to NE was similar for both methods; however, for aorta from SHR, the reactivity obtained by cumulative-dose method was significantly higher. Furthermore, the cumulative-dose method yielded higher sensitivity in both WKY and SHR when compared with that obtained by single-dose method. Taken together, the cumulative-dose method appeared to be a more sensitive method to determine contractile response. Nevertheless, no significant difference in either reactivity or sensitivity to NE in aorta was detected between WKY and SHR when cumulative-dose method was employed. These data indicate that aorta do not exhibit abnormal response to NE in SHR, consistent with earlier findings that not all tissue preparations behave similarly.