Empirically determined freezing time for quick-freezing with a liquid-nitrogen-cooled copper block
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1Departement de Physiologie générale, Université Catholique de Louvain, Brussels, Belgium.
Journal of Microscopy
|October 1, 1993
Abstract:
A method is presented to determine freezing time empirically. The method is based on determining the amount of stretch of a skinned muscle fibre while it is being frozen. Freezing time, as determined with this method lies in between 0.5 and 1.5 ms.