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Related Experiment Videos

Simplified nerve cell counting in the rat brainstem with the physical disector using a drawing-microscope

O Guntinas-Lichius1, J Mockenhaupt, E Stennert

  • 1Department of Anatomy, University of Cologne, Germany.

Journal of Microscopy
|November 1, 1993
PubMed
Summary

This study presents a simple physical disector modification for counting rat hypoglossal nucleus neurons. The method uses two adjacent Nissl-stained sections and a drawing microscope for accurate cell enumeration.

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Area of Science:

  • Neuroscience
  • Stereology
  • Histology

Background:

  • Accurate neuron counting is crucial for understanding brain structure and function.
  • Stereological methods, like the physical disector, are standard for unbiased cell enumeration.
  • Existing methods may require specialized equipment or complex procedures.

Purpose of the Study:

  • To present a simplified, accessible modification of the physical disector method.
  • To enable accurate counting of neurons in the rat hypoglossal nucleus.
  • To offer a technique adaptable for combined histochemical studies.

Main Methods:

  • A modified physical disector using two adjacent 6-micrometer Nissl-stained paraffin sections.
  • Utilizing a light microscope with a drawing apparatus for marking neuronal profiles.

Related Experiment Videos

  • Overlaying drawings from serial sections to count neurons present in one but not both sections.

    • Main Results:

    • The modified disector method provides a straightforward approach to neuron counting.
    • The technique requires no specialized equipment beyond standard microscopy.
    • Demonstrated applicability for quantifying neurons in the rat hypoglossal nucleus.

    Conclusions:

    • This modified physical disector offers a practical and cost-effective method for neuron enumeration.
    • The technique is suitable for researchers with basic microscopy facilities.
    • The method's compatibility with histochemistry allows for integrated neuroanatomical research.