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Simplified nerve cell counting in the rat brainstem with the physical disector using a drawing-microscope
O Guntinas-Lichius1, J Mockenhaupt, E Stennert
1Department of Anatomy, University of Cologne, Germany.
Journal of Microscopy
|November 1, 1993
Summary
This study presents a simple physical disector modification for counting rat hypoglossal nucleus neurons. The method uses two adjacent Nissl-stained sections and a drawing microscope for accurate cell enumeration.
Area of Science:
- Neuroscience
- Stereology
- Histology
Background:
- Accurate neuron counting is crucial for understanding brain structure and function.
- Stereological methods, like the physical disector, are standard for unbiased cell enumeration.
- Existing methods may require specialized equipment or complex procedures.
Purpose of the Study:
- To present a simplified, accessible modification of the physical disector method.
- To enable accurate counting of neurons in the rat hypoglossal nucleus.
- To offer a technique adaptable for combined histochemical studies.
Main Methods:
- A modified physical disector using two adjacent 6-micrometer Nissl-stained paraffin sections.
- Utilizing a light microscope with a drawing apparatus for marking neuronal profiles.
- Overlaying drawings from serial sections to count neurons present in one but not both sections.
Main Results:
- The modified disector method provides a straightforward approach to neuron counting.
- The technique requires no specialized equipment beyond standard microscopy.
- Demonstrated applicability for quantifying neurons in the rat hypoglossal nucleus.
Conclusions:
- This modified physical disector offers a practical and cost-effective method for neuron enumeration.
- The technique is suitable for researchers with basic microscopy facilities.
- The method's compatibility with histochemistry allows for integrated neuroanatomical research.