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Crystallization and preliminary crystallographic data of vitelline membrane outer layer protein I, VMO-I
T Shimizu1, K Morikawa, S Kido
1Protein Engineering Research Institute, Osaka, Japan.
Journal of Molecular Biology
|January 14, 1994
Abstract:
The vitelline membrane outer layer protein I (VMO-I), which is isolated from the vitelline membrane outer layer of hen's eggs, has been crystallized from an acetate buffer solution by the hanging-drop method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions a = 62.42 A, b = 110.52 A, c = 44.15 A. There are two molecules (M(r) = 18,000) per asymmetric unit. The crystals diffract to at least 2.2 A Bragg spacings.