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DNA sequence organization in chromatosomes

S Muyldermans1, A A Travers

  • 1Vrije Universiteit Brussel, Instituut Voor Moleculaire Biologie, St. Genesius-Rode, Belgium.

Journal of Molecular Biology
|January 21, 1994
PubMed
Summary
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Chromatosomes and nucleosome core particles share similar DNA rotational positioning around histone octamers. Linker histone binding influences DNA sequence organization, favoring G+C-rich regions and excluding oligo(dA).(dT) tracts.

Area of Science:

  • Chromatin biology
  • Molecular genetics
  • Structural biology

Background:

  • The chromatosome is a fundamental structural unit of chromatin, comprising a histone octamer and a linker histone bound to DNA.
  • Understanding DNA organization within chromatosomes is crucial for elucidating gene regulation and chromatin structure.

Purpose of the Study:

  • To investigate the DNA sequence organization and rotational positioning within chicken erythrocyte chromatosomes.
  • To compare DNA characteristics in chromatosomes with those in nucleosome core particles.

Main Methods:

  • Cloning and sequencing of 280 DNA fragments (163-173 bp) isolated from chicken erythrocyte chromatosomes.
  • Analysis of trinucleotide sequence frequencies and periodic modulations.
  • Fourier analysis to assess rotational positioning of DNA.

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Main Results:

  • Rotational positioning of DNA around the histone octamer is conserved between chromatosomes and core particles.
  • Linker histone binding modulates DNA sequence preferences, favoring G+C-rich sequences and excluding oligo(dA).(dT) tracts.
  • Specific base-steps are enriched near chromatosomal DNA termini, potentially involved in linker histone binding.

Conclusions:

  • The rotational positioning of DNA is largely maintained in chromatosomes, similar to core particles.
  • Linker histones influence DNA sequence organization, overriding some intrinsic DNA bending preferences.
  • Identified sequence features suggest specific binding sites for linker histone globular domains.