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Lymphocytic proliferative response to outer-membrane proteins isolated from Salmonella
C R González1, A Isibasi, V Ortiz-Navarrete
1Laboratorio de Inmunoqúimica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, D.F. México.
Microbiology and Immunology
|January 1, 1993
Summary
Salmonella porins and outer-membrane proteins (OMPs) induce significant T cell responses in mice, suggesting a role for T cell activation in protective immunity against bacterial infections.
Area of Science:
- Immunology
- Microbiology
- Bacterial Pathogenesis
Background:
- Porins from Salmonella typhi offer protection against bacterial challenge in mice.
- The exact mechanism of protection, potentially involving humoral and cellular immunity, remains unclear.
Purpose of the Study:
- To investigate the induction of specific T cell responses to porins from Salmonella typhimurium, Salmonella typhi, and Escherichia coli.
- To evaluate the role of T cell activation in porin-mediated protective immunity.
Main Methods:
- Mice were immunized with acetone-killed S. typhimurium, S. typhimurium porins, or S. typhi outer-membrane proteins (OMPs).
- Lymphocytic proliferation was assessed using a 3H-thymidine incorporation assay.
- Cross-reactivity and mitogenic potential of porins were examined.
Main Results:
- Immunization with porins and OMPs elicited higher proliferative responses compared to whole bacteria.
- Porins showed cross-reactivity but were not mitogenic.
- Porins activated T lymphocytes from mice immunized with S. typhi OMPs.
Conclusions:
- T cell activation, likely via lymphokine release, contributes to the protective immunity induced by porins.
- Porins are potent inducers of T cell-mediated immune responses relevant to Salmonella infections.