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[Isolation of the mRNA for interferon and its integration into the genome of a foreign host]
Abstract:
The study of superinduction and translation of interferon mRNA (mRNA-IF) in four cell systems was the experimental basis for tests on the establishment in mouse and monkey cells (L-1210 and BSC) of the capacity for long-term production of biologically active chicken interferon after their inoculation with chicken mRNA-IF. The interferon was tested and identified by the species-specificity, thermostability and antigenic specificity. During 3 months (the observation period) after a single inoculation of chicken mRNA-IF the L-1210 and BSC cells in response to induction with poly: C produced the homologous (mouse or monkey) and heterologous (chicken) interferons without any signs of decline of the latter's production. Titres of the heterologous interferon exceeded those of homologous interferon by approximately 10-fold. The dynamics of production of chicken interferon after induction and the sensitivity of this process to actinomycin D permit a conclusion on similar mechanisms of transcription and translation of homologous and heterologous interferons. It is suggested that integration of the genetic information for synthesis of chicken interferon with the genome of heterogenous cells may be a possible mechanism of this phenomenon.
Insights
Chicken cells can produce biologically active interferon in mouse and monkey cells long-term. This interferon production, even exceeding native levels, suggests similar gene expression mechanisms and potential genetic integration.
Area of Science:
- Molecular Biology
- Virology
- Immunology
Background:
- Interferon mRNA (mRNA-IF) translation and superinduction were studied in various cell systems.
- The capacity for sustained interferon production in heterologous cells was investigated.
Purpose of the Study:
- To establish long-term production of biologically active chicken interferon in mouse and monkey cells.
- To investigate the mechanisms governing homologous and heterologous interferon production.
Main Methods:
- Inoculation of L-1210 (mouse) and BSC (monkey) cells with chicken mRNA-IF.
- Induction of interferon production using poly:C.
- Characterization of interferon by species-specificity, thermostability, and antigenic specificity.
- Assessment of production dynamics and sensitivity to actinomycin D.
Main Results:
- Mouse and monkey cells produced biologically active chicken interferon for 3 months post-inoculation.
- Heterologous (chicken) interferon titers were approximately 10-fold higher than homologous (mouse/monkey) interferon.
- Interferon production dynamics and actinomycin D sensitivity indicated similar transcription/translation mechanisms for both types of interferon.
Conclusions:
- Chicken interferon can be produced long-term in mouse and monkey cells, with titers exceeding homologous interferon.
- Transcription and translation mechanisms for homologous and heterologous interferons appear similar.
- Genetic information integration into host cell genomes is a potential mechanism for sustained heterologous interferon synthesis.