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Updated: Oct 3, 2026

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Published on: December 3, 2007
[General principles of the production and use of retroviral vectors]
1Laboratoire de Biochimie Génétique, Université René Descartes, CHU Paris-Ouest, Hôpital Robert Debré, France.
Abstract:
This paper describes the various strategies for somatic gene therapy. There are two main scenarios: the ex vivo approach and the in vivo approach. As recombinant retroviruses were used in the first clinical trials of gene therapy, their main characteristics (structure, efficient production of helper-free viral stocks by using packaging cells, transduction procedures) are discussed as well as the related safety issues. Finally, retroviral vectors can also be used as a new tool to investigate various clinical situations as they allow to mark cells by stably integrating in the cellular genome.
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