Related Experiment Video
Updated: Aug 8, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Regulation of Arabidopsis thaliana L. (Heyn) cor78 in response to low temperature
D P Horvath1, B K McLarney, M F Thomashow
1Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Abstract:
Changes in gene expression occur during cold acclimation in a variety of plants including Arabidopsis thaliana L. (Heyn). Here we examine the cold-regulated expression of A. thaliana cor78. The results of gene-fusion experiments confirm the finding of Yamaguchi-Shinozaki and Shinozaki ([1993] Mol Gen Genet 236: 331-340) that the 5' region of cor78 has cis-acting regulatory elements that can impart cold-regulated gene expression. Further, histochemical staining experiments indicated that this cold-regulatory element(s) was active at low temperature throughout much of the plant including leaves, stems, roots, flower petals, filaments, and sepals. Time-course experiments indicated that the activity of the cor78 promoter in cold-acclimated plants was down-regulated quickly in response to noninducing temperatures and that the half-life of the cor78 transcripts was only about 40 min at normal growth temperature. Fusion of the entire transcribed region of cor78 to the cauliflower mosaic virus 35S promoter resulted in a chimeric gene that was constitutively expressed and displayed little if any posttranscriptional regulation in response to low temperature.
Related Concept Videos
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Regulation of Transpiration by Stomata
Biological Clocks and Seasonal Responses
Responses to Heat and Cold Stress
Cell Signaling in Plants
Gene Regulation During Sporulation

