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Detection of Cryptosporidium parvum using a specific polymerase chain reaction
K A Webster1, J D Pow, M Giles
1Central Veterinary Laboratory, New Haw, Weybridge, UK.
Veterinary Parasitology
|October 1, 1993
Abstract:
The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.