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Preservation time dependent morphological changes in cold stored human donor pancreas
P In't Veld1, H Nizze, G Klöppel
1Department of Pathology, Free University of Brussels VUB, Belgium.
Virchows Archiv. A, Pathological Anatomy and Histopathology
|January 1, 1993
Summary
For successful pancreatic transplantation, donor pancreases should be cold preserved for less than 24 hours. Longer preservation times damage acinar cells, compromising graft function.
Area of Science:
- Transplantation immunology
- Organ preservation
- Diabetes mellitus research
Background:
- Pancreatic transplantation is a key treatment for insulin-dependent diabetes.
- Graft structural integrity is essential for successful pancreatic function.
- Understanding organ preservation effects is vital for transplant outcomes.
Purpose of the Study:
- To evaluate light microscopic and ultrastructural changes in cold-preserved human donor pancreases.
- To determine the optimal cold preservation time for pancreatic grafts.
- To identify specific cell types vulnerable to ischemic damage during preservation.
Main Methods:
- Analysis of 30 human donor pancreases stored in University of Wisconsin solution.
- Categorization of storage times: <24 hours (n=23) and >30 hours (n=7).
- Microscopic and ultrastructural examination of pancreatic tissue.
Main Results:
- Pancreases stored >30 hours showed cytoplasmic vacuolization in acinar cells.
- Grafts stored >40 hours exhibited focal acinar necrosis.
- Endocrine and duct cells demonstrated minimal to no significant changes.
Conclusions:
- Cold-preserved pancreases are optimal for transplantation when stored for <24 hours.
- Pancreatic acinar cells are more susceptible to ischemic injury than endocrine or duct cells.
- Preservation time significantly impacts pancreatic graft viability and function.