Related Experiment Video
Updated: Jul 27, 2026
Facile Preparation of 4-Substituted Quinazoline Derivatives
Published on: February 15, 2016
A simplified gas chromatographic method for quantifying the sevoflurane metabolite hexafluoroisopropanol
S E Morgan1, E J Frink, A J Gandolfi
1Department of Anesthesiology, University of Arizona, Tucson.
Background:
The results of sevoflurane biotransformation (fluoromethyl-1,1,1,3,3,3,-hexafluoro-2-propyl ether) to inorganic fluoride have been examined. However, these investigations have lacked a simplified assay for determining the primary organic metabolite, hexafluoroisopropanol. Previous attempts have involved extensive extraction steps, complicated derivatization techniques, or sophisticated detectors.
Methods:
After enzymatic hydrolysis of conjugates, hexafluoroisopropanol is detected readily using a head space gas chromatographic analysis with a flame ionization detector.
Results:
The gas chromatographic technique was linear from 10 to 800 microM with a correlation coefficient of 0.999. The detection limit was 10 microM in urine and 25 microM in blood.
Conclusions:
This simplified approach does not require the extraction, derivatization, or mass spectrometric detectors of previous methods. As sevoflurane utilization and research increases, this assay should allow for a variety of laboratory and clinical disposition studies to be performed.
