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Updated: Aug 8, 2026

Single-Molecule Imaging of Nuclear Transport
Published on: June 9, 2010
Pure thoughts with impure proteins: permeabilized cell models of organelle motility
1Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
Abstract:
Permeabilized cell models provide an experimental middle ground wherein the in vitro properties of mechanochemical proteins can be reconciled with the physical and topological constraints of the intact cell. Several well-studied examples of organelle motility are described here, including the actin-based cytoplasmic streaming of Characean algae, the microtubule-based aggregation and dispersion of pigment granules in chromatophores and the saltatory movements of vesicles along microtubules in fibroblasts and macrophages. The permeabilized models developed for these systems have helped to integrate observations in vivo with in vitro assays of motor proteins.
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