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The cGMP-inhibitable phosphodiesterase modulates glucose transport activation by insulin

J W Eriksson1, C Wesslau, U Smith

  • 1Department of Medicine, University of Göteborg, Sahlgren's Hospital, Sweden.

Biochimica Et Biophysica Acta
|January 19, 1994
PubMed

Insights

The cGMP-inhibitable phosphodiesterase (cGI-PDE) modulates insulin

Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolic Research

Background:

  • Insulin is crucial for glucose uptake in adipocytes.
  • The role of cGMP-inhibitable phosphodiesterase (cGI-PDE) in insulin signaling is not fully understood.
  • Elevated cAMP levels can influence insulin sensitivity.

Purpose of the Study:

  • To investigate the role of cGI-PDE in insulin-stimulated glucose transport.
  • To determine if cGI-PDE inhibition affects insulin sensitivity, particularly under conditions of elevated cAMP.
  • To examine cGI-PDE activity in insulin-resistant states.

Main Methods:

  • Adipocytes from young, lean rats were used.
  • Cells were treated with OPC 3911, a specific cGI-PDE inhibitor.
  • 3-O-methylglucose uptake was measured with and without insulin stimulation.
  • The effects of OPC 3911 were assessed alone and in combination with 8-bromo-cAMP, a cAMP analogue.

Main Results:

  • OPC 3911 impaired insulin-stimulated glucose transport by approximately 15%.
  • This impairment was significantly greater (approximately 45%) when combined with 8-bromo-cAMP.
  • Insulin sensitivity was reduced by the combination of OPC 3911 and 8-bromo-cAMP.
  • In aging, obese rats, maximal insulin-stimulated glucose transport was similarly affected by OPC 3911 and 8-bromo-cAMP, suggesting no significant change in cGI-PDE activity in this insulin-resistant state.

Conclusions:

  • cGMP-inhibitable phosphodiesterase (cGI-PDE) plays a modulating role in insulin's effect on glucose transport.
  • This modulation is more pronounced when cellular cyclic adenosine monophosphate (cAMP) levels are elevated.
  • The cGI-PDE pathway does not appear to be significantly altered in the insulin resistance associated with aging and obesity.

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