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An in vivo Assay to Test Blood Vessel Permeability
Published on: March 16, 2013
Measurement of cutaneous microvascular exudates using Evans blue
1Department of Pathology, State University of New York Health Science Center at Syracuse.
Summary
Researchers developed a precise method to quantify plasma leakage in skin using Evans blue dye (EB). This technique enhances the study of inflammation and allows for parallel biochemical analysis.
Area of Science:
- Pharmacology
- Physiology
- Biochemistry
Background:
- Inflammation involves increased vascular permeability and plasma leakage.
- Accurate quantification of plasma exudate is crucial for studying inflammatory responses.
- Current methods may have limitations in precision and scope.
Purpose of the Study:
- To evaluate strategies for the quantitative assessment of plasma exudate.
- To establish a reliable method for measuring plasma leakage in cutaneous microvasculature.
- To demonstrate the feasibility of integrating biochemical studies.
Main Methods:
- Measurements were based on the recovery of Evans blue dye (EB) from rat skin.
- Tissue homogenization in an acetone, water, and sodium sulfate mixture optimized EB recovery.
- Results were expressed as agonist-induced plasma accumulation for precise quantification.
Main Results:
- An optimized extraction method achieved almost complete recovery of Evans blue dye.
- Identifying and controlling sources of variation led to precise EB measurements.
- The developed method demonstrated feasibility for parallel biochemical investigations.
Conclusions:
- A robust and precise method for quantifying plasma exudate in skin was established.
- This quantitative approach using Evans blue dye aids in the study of inflammation.
- The method supports integrated physiological and biochemical investigations of inflammatory processes.
