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Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen
L S Robert1, S Allard, J L Gerster
1Plant Research Centre, Centre Experimental Farm, Ottawa, Ontario, Canada.
Plant Molecular Biology
|December 1, 1993
Abstract:
A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars.