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Stroma-free haemoglobin solution for infusion: changes during storage

M Kramlová, T I Pristoupil, S Ulrych

    Haematologia
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    Freezing haemoglobin solution for infusion (HSI) is the most effective method for maintaining its stability for up to one year. Other storage conditions like refrigeration or room temperature led to significant degradation.

    Area of Science:

    • Biochemistry
    • Biotechnology
    • Materials Science

    Background:

    • Haemoglobin solution for infusion (HSI) is a potential blood substitute.
    • Ensuring the long-term stability of HSI is crucial for its clinical application.
    • Understanding degradation pathways under various storage conditions is essential.

    Purpose of the Study:

    • To investigate the stability of experimental haemoglobin solution for infusion (HSI) over a one-year storage period.
    • To evaluate the impact of different storage temperatures and conditions on HSI integrity.
    • To identify optimal storage conditions for preserving HSI injectability.

    Main Methods:

    • Storage of HSI samples at various temperatures: 20-25°C, 4°C, -8 to -12°C, and -195.8°C for one year.
    • Monitoring key stability parameters: precipitate formation, oxidation to methemoglobin, and changes in absorption spectrum (550-650 nm).

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  • Testing the efficacy of stabilizers (EDTA, ascorbic acid, glutathione) and nitrogen atmosphere storage.

    • Main Results:

    • Negligible changes observed in HSI samples stored frozen (-8 to -195.8°C), indicating excellent stability.
    • Significant increase in methemoglobin and spectral changes occurred in HSI stored at 4°C.
    • Profound degradation of HSI parameters was observed at room temperature (20-25°C).
    • Tested additives and nitrogen atmosphere did not improve HSI stability under tested conditions.

    Conclusions:

    • Cryopreservation (freezing) is the most feasible method for maintaining HSI injectability for at least one year.
    • Refrigeration and room temperature storage lead to unacceptable degradation of HSI.
    • Further research may be needed to identify effective stabilizers for non-frozen HSI storage.