Peripheral adaptations in trained aged rats with femoral artery stenosis

The development and functional significance of exercise-induced peripheral adaptations were evaluated in aged animals with peripheral arterial insufficiency. Fisher 344 male rats (21 months old) were subjected to bilateral stenosis of the femoral arteries sufficient to limit active hyperemia but not to impair resting blood flow. Beginning the third day after stenosis, animals were (1) exercised by walking (n = 12) on a treadmill at 20 m/min at 15% inclination, twice a day, 5 days per week, or (2) limited to cage activity (n = 10). Exercise tolerance improved from approximately 5 to approximately 35 minutes (P < .001) over the 8 weeks of the training program but increased only marginally to approximately 8 minutes for the sedentary group. An isolated hind limb preparation perfused at equivalent blood flows (approximately 1 ml.min-1 x g-1 with an arterial blood oxygen content of approximately 20 vol%) was used to assess the functional and metabolic impact of muscle-specific adaptations during sequential contraction periods at 4, 8, 15, 30, 45, 60, 75, and 90 tetani per minute. An initially similar force development of approximately 10 N/g was better maintained (P < .001) by the trained group. The peak oxygen consumption attained by the trained group of 5.68 +/- 0.34 mumol.min-1 x g-1 was greater (P < .01) than that of the sedentary group (4.34 +/- 0.29 mumol.min-1 x g-1). This was due to a greater oxygen extraction, since oxygen delivery was the same (approximately 10 mumol.min-1 x g-1) to muscles of both groups.(ABSTRACT TRUNCATED AT 250 WORDS)