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Analysis of the influence of chromosomal condensation on the activity of restriction endonucleases
A M González-Tizón1, A Martínez-Lage, J Méndez
1Departamento de Biología Celular y Molecular, Area de Genética, Universidad de la Coruña, Spain.
Cytobios
|January 1, 1993
Abstract:
The different responses of metaphase chromosomes to restriction endonuclease activity depend on the stage of chromosomal condensation. Results obtained established that this factor plays a remarkable role in obtaining high resolution banding patterns which facilitated visualization of a large number of sub-bands. It is evident that various restriction endonucleases, which are able to induce a coincident G-like banding pattern on Don cell line chromosomes, act in very different ways.