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Comparison of the binding sites of plant ferredoxin for two ferredoxin-dependent enzymes
A R De Pascalis1, P Schürmann, H R Bosshard
1Biochemisches Institut, Universität Zürich, Switzerland.
FEBS Letters
|January 17, 1994
Abstract:
Differential chemical modification of acidic residues was used to map the binding site of plant ferredoxin (Fd) for the chloroplast enzyme ferredoxin:thioredoxin reductase (FTR). Binding of FTR to Fd inhibits chemical modification of Fd residues D34, D65, E92, E93, E94 and C-terminal A97. The binding site demarcated by these residues differs from that for ferredoxin:NADP+ reductase (FNR). The FTR site includes C-terminal residues but not helix 24-31, which is part of the FNR site. Both sites enclose the [2Fe-2S] cluster.