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Salivary excretory pattern of testosterone in substitutive therapy with testosterone enanthate
M A Navarro1, C M Villabona, A Blanco
1Hormone Unit (Biochemistry Service), Ciutat Sanitària i Universitària de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
Objective:
To determine salivary and serum T levels by an RIA method after a single injection of 250 mg IM of commercially available T enanthate.
Design:
Research study.
Setting:
Patients attended in a hospital environment.
Patients:
Sixteen men with secondary hypogonadism.
Interventions:
Testosterone enanthate was administered, and salivary samples were taken before the injection. Thereafter, these samples were obtained daily until day 7 and then on alternate days until day 28 after injection. Blood samples were taken previously and after the injection (5 samples during the month).
Main Outcome Measure:
Salivary and serum T.
Results:
Salivary T levels rose from 3.46 +/- 3.16 to 13.82 +/- 7.78 ng/100 mL (0.12 +/- 0.11 to 0.48 +/- 0.27 nmol/L) within 24 hours and remained in that range until day 7. From day 9, 7.20 +/- 2.88 ng/100 mL (0.25 +/- 0.10 nmol/L), a progressive decrease of these values was observed until day 14: 5.18 +/- 2.88 ng/100 mL (0.18 +/- 0.10 nmol/L). From day 16 until day 28, salivary concentrations returned to hypogonadal levels.
Conclusion:
We conclude that T salivary concentrations may be applied to assess the effectiveness of T substitutive therapy in hypogonadal men and could replace serum in the monitoring of this type of therapy.