Subcloning and restriction enzyme mapping of bovine adenovirus type 2

Bovine adenoviruses (BAVs), causing both respiratory and/or enteral diseases in cattle, have been isolated in many countries all over the world. In this study we report on the molecular cloning of the internal EcoRI fragments spanning 20.6-90.5%, and the internal SalI fragments spanning 3.1-65.2% of the BAV type 2 (BAV2; strain No. 19) genome into the plasmid vector pUC19. Moreover, the subcloning of the BAV2 genome facilitated the construction of a detailed physical restriction endonuclease map for BamHI, ClaI, EcoRI, HindIII, KpnI, NotI, NspV, PstI, PvuI, SalI, XbaI and XhoI.