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Updated: Oct 5, 2026

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Published on: August 9, 2017
Effect of benzo[a]phenothiazines on natural killer cell activity of human peripheral blood mononuclear cells
I B Petri1, E Szekeres, I Berek
1Blood Transfusion Centre, Szeged, Hungary.
Abstract:
Four benzo[a]phenothiazines were investigated for their effects on antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) cell and blast transformation of human peripheral blood mononuclear cells (PBM). Benzo[a]phenothiazines dose-dependently suppressed the nitrogen-stimulated T cell proliferation. The suppressive effect of non differentiation-inducing benzo[a]phenothiazines was higher than that of differentiation-inducing benzo[a]phenothiazines. The stimulation effect of differentiation-inducing compounds on the NK activity was reduced by removal of monocytes from the PBM. The results suggest the direct activation of monocytes (Mo) by differentiation-inducing benzo[a]phenothiazines.

