Related Experiment Videos
Quantitative measurement of hemosiderin deposition in tissue sections of the liver by image analysis
T Masuda1, T Kasai, R Satodate
1Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan.
Analytical and Quantitative Cytology and Histology
|December 1, 1993
Abstract:
Hemosiderin has been quantitated in tissue sections by image analysis employing Perls' Berlin blue reaction. A linear correlation was observed between the mean optical density of the tissue section measured with a commercial image analyzer and the iron concentration measured with an atomic absorption spectrophotometer. The correlation coefficient of .945 (P < .001) indicated that a linear correlation existed between these measurements. Thus, the hemosiderin volume in tissue sections can be measured quantitatively by image analysis.