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X-ray microanalysis of guinea pigs peripheral blood T and B lymphocytes: before and after antigen stimulation
1Laboratory of Electron Microscopy, Military Medical Academy, Sofia, Bulgaria.
T and B lymphocytes from with Francisella tularensis (LVS) immunized guinea pigs were separated and stimulated in vitro with the specific antigen. Their chemical composition was studied in comparison by X-ray microanalysis before and after stimulation. Different elemental spectra of normal T, B and T-suppressor cells were obtained. The common elements were P, S, K, Cl and As; I was present only in B cells; Cr and Na were detected only in T suppressor cells. After stimulation the spectra of these three types of lymphocytes differed both from the original normal ones and in between. I disappeared in the spectrum of B cells but the peaks of P and Cl raised up; the peaks of S and P were higher in the activated T cells along with the appearance of Na and Si; an increase of the peak of Cl in the spectrum of T suppressor cells was revealed on the 21st day after the immunization. The registered differences in the chemical composition of the stimulated lymphocyte populations are possibly connected with the different type of response to the antigen stimulation.
T and B lymphocytes from with Francisella tularensis (LVS) immunized guinea pigs were separated and stimulated in vitro with the specific antigen. Their chemical composition was studied in comparison by X-ray microanalysis before and after stimulation. Different elemental spectra of normal T, B and T-suppressor cells were obtained. The common elements were P, S, K, Cl and As; I was present only in B cells; Cr and Na were detected only in T suppressor cells. After stimulation the spectra of these three types of lymphocytes differed both from the original normal ones and in between. I disappeared in the spectrum of B cells but the peaks of P and Cl raised up; the peaks of S and P were higher in the activated T cells along with the appearance of Na and Si; an increase of the peak of Cl in the spectrum of T suppressor cells was revealed on the 21st day after the immunization. The registered differences in the chemical composition of the stimulated lymphocyte populations are possibly connected with the different type of response to the antigen stimulation.