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Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Published on: October 13, 2014
Identification of the metabotropic glutamate receptor-1 protein in the rat trigeminal ganglion
T Araki1, J G Kenimer, A Nishimune
1Department of Anatomy and Neuroscience, Osaka University Medical School, Japan.
Brain Research
|November 12, 1993
Abstract:
Anti-metabotropic glutamate receptor-1 monoclonal antibody was raised and applied for immunohistochemistry in the rat trigeminal ganglion. The antibody detected 145-kDa single band of protein in the immunoblot analysis. In immunohistochemistry, neurons in the trigeminal ganglion showed immunostaining with various intensity, almost irrespective of their cell size. The results indicate that metabotrophic glutamate receptors play an important role in somatic sensation together with ionotropic ones.
