Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons
Related Experiment Video
Updated: Nov 8, 2026

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
Published on: December 2, 2022
J Polaina1, A C Adam, L del Castillo
1Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain.
Abstract:
Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type, about 10% of the cytoductants of both mating types are homozygous autodiploids. There is evidence indicating that self-diploidization occurs by fusion between sibling nuclei in the heterokaryotic zygote. Here we describe this phenomenon and propose to take advantage of it for the construction of genotypically-defined diploids able to mate, and of polyploid strains, which are useful tools in genetic and cytological studies.
Related Concept Videos
Meiosis II
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Yeast Signaling
Meiosis I
Crossing Over

