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Esterases in quail (Coturnix coturnix). Physicochemical and developmental aspects

O E Tsitsiloni1, M Veini, A A Haritos

  • 1Zoological Laboratory, University of Athens, Faculty of Science, Panepistimiopolis, Greece.

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
|December 1, 1993
PubMed
Summary

Soluble esterases in quail digestive organs show diverse molecular forms. Their expression is specific to tissue type and developmental stage, becoming more complex after hatching.

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Comparative Physiology

Background:

  • Esterases are crucial enzymes involved in various physiological processes.
  • Understanding esterase diversity and expression is key to comprehending metabolic regulation.
  • Quail (Coturnix coturnix) serve as a valuable model for avian developmental studies.

Purpose of the Study:

  • To characterize the soluble esterases in digestive organs of quail across different developmental stages.
  • To investigate the tissue-specific and developmental-stage-specific expression of esterase molecular forms.
  • To compare esterase profiles between quail and chicken tissues.

Main Methods:

  • Polyacrylamide gel electrophoresis (PAGE) was used to resolve esterase isozymes.

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  • Isoelectric focusing (IEF) was employed to determine the isoelectric point (pI) of esterases.
  • Esterase activity was characterized based on substrate specificity and inhibitor sensitivity (e.g., eserine).

    • Main Results:

    • Multiple molecular forms of soluble esterases were identified, including carboxylesterases, acetylesterases, and cholinesterases.
    • The majority of esterase activity exhibited an isoelectric point (pI) between 5.1-5.6.
    • Apparent molecular weight of liver esterases was approximately 60,000 Da.
    • Esterase expression patterns were distinct for different tissues and developmental stages.
    • Electrophoretic patterns became more complex post-hatching, particularly in liver and intestinal tissues.

    Conclusions:

    • Quail possess a diverse array of soluble esterases in their digestive system.
    • Esterase expression is tightly regulated, varying with tissue type and developmental progression.
    • These findings provide insights into the enzymatic machinery supporting digestion and metabolism in developing birds.