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Production of interleukin-1 by primary cultured parenchymal liver cells (hepatocytes)
T Tsukui1, K Kikuchi, A Mabuchi
1Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Experimental Cell Research
|February 1, 1994
Abstract:
The production of interleukin-1 (IL-1) by cultured parenchymal liver cells was revealed by a biological assay with an IL-1-dependent cell line, Northern blot analysis, and in situ hybridization. Inhibition experiments on the IL-1 activity with anti IL-1 alpha antibody also support the presence of IL-1 alpha in the supernatant of cultured parenchymal liver cells. Based on these results, we discuss the possibility of IL-1 production by parenchymal liver cells in vivo.