[Development and application possibilities of a computer-controlled microspectrophotometric system for cytoanalysis...

A computer-assisted microspectrophotometric system for quantitative cell identification and data conversion, based on the scanned intracellular image pattern, will be introduced. The CYTOS system was developed with high flexibility for the analysis of general morphological and physiological problems of cell recognition and classification. The investigation include the quantitative transformation of extinction values in relation to cytomorphologic and cytochemical properties of a cell. In order to objectivate the subjective impression of visible or non-visible information, contained in a microscopic image, the measured extinction values were transformed in color values by a special developed interface. Each color shade corresponds to the intracellular substance concentration. The results, presented, concentrate on the CYTOS methodology, its application to the translation of data cytograms of DNA containing chromatic structures, and the intra-cytoplasmatic distribution pattern of enzyms. The CYTOS method might be considered as a useful technique revealing discrimination factors of diagnostical value.