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Related Experiment Videos

Hypercoagulable state induced by thrombocytapheresis

I Kobayashi1, S Hamaoka, H Ozawa

  • 1Second Department of Internal Medicine, Yamanashi Medical College, Japan.

Journal of Clinical Apheresis
|January 1, 1993
PubMed
Summary

Platelet pheresis using the Fenwal CS-3000 cell separator induces a hypercoagulable state in blood donors. Thromboresistant materials may prevent this effect.

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Area of Science:

  • Hematology
  • Transfusion Medicine
  • Biomaterials Science

Background:

  • Platelet pheresis is a common procedure for blood donation.
  • Automated cell separators are widely used in apheresis procedures.
  • The impact of extracorporeal circulation on coagulation and fibrinolysis requires further investigation.

Purpose of the Study:

  • To investigate the effects of the Fenwal CS-3000 continuous-flow automated blood cell separator on blood coagulation and fibrinolysis in blood donors.
  • To identify molecular markers indicative of coagulation changes during platelet pheresis.
  • To explore potential strategies for mitigating adverse coagulation effects.

Main Methods:

  • Blood samples were collected from donors undergoing platelet pheresis using the Fenwal CS-3000.

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  • Analysis of molecular markers including prothrombin fragment F1+2 (PF1+2), thrombin-antithrombin III complex (TAT), fibrinopeptide A (FPA), and alpha 2-plasmin inhibitor (α2-PI).
  • Assessment of coagulation factors and inhibitors in a donor exhibiting the highest marker levels.

    • Main Results:

    • Significant increases in PF1+2 (0.8 to 2.9 nM/ml) and TAT (2.6 to 56.0 µg/L) were observed.
    • Slight increases in FPA (0.8 to 3.8 µg/L) and decreases in α2-PI (95% to 91%) were noted.
    • A hypercoagulable state was evident, with decreased levels of various coagulation factors and inhibitors in one donor.

    Conclusions:

    • The Fenwal CS-3000 automated blood cell separator induces a hypercoagulable state during platelet pheresis.
    • Thromboresistant materials for plastic disposables may prevent donor hypercoagulability.
    • Further research into biomaterial improvements is warranted to enhance donor safety during apheresis.