Related Experiment Videos
Determination of plasma tocopherols by high-performance liquid chromatography with coulometric detection
C Sarzanini1, E Mentasti, M Vincenti
1Department of Analytical Chemistry, University of Torino, Italy.
Journal of Chromatography
|October 29, 1993
Abstract:
An HPLC procedure has been developed for tocopherol determination with coulometric detection in human serum samples. Eluent optimization and foreign peak identification (bilirubin) by mass spectrometry are described. An extraction procedure gave yields around 98% with 1.3% coefficient of variation, and the calibration ranged from 0.1 to 200 mg/l with a correlation coefficient of 0.999. The detection limit achieved for vitamin E was 60 pg (3 ng/ml).