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Related Experiment Videos

The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex

S F Ziegler1, S D Levin, L Johnson

  • 1Immunex Research and Development Corporation, Seattle, WA 98101.

Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1994
PubMed
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CD69, a T cell activation antigen, is rapidly and transiently induced in T cells. Its gene structure and chromosomal location in mice and humans were analyzed, revealing links to immune gene clusters and leukemia.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • CD69 is an early activation antigen on T cells and other hematopoietic cells.
  • It is a type II membrane glycoprotein belonging to the C-type lectin family.

Purpose of the Study:

  • To investigate the kinetics of CD69 mRNA induction in activated T cells.
  • To analyze the structure and chromosomal localization of the mouse and human CD69 genes.

Main Methods:

  • Murine thymocytes and T cells were stimulated, and CD69 mRNA levels were measured over time.
  • Mouse CD69 gene structure was analyzed, including promoter elements.
  • Chromosomal mapping was performed for both mouse and human CD69 genes.

Main Results:

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  • CD69 mRNA induction in activated murine T cells was rapid (peaking at 30-60 min) and transient (decreasing by 8 h).
  • The mouse CD69 gene has 5 exons with a phorbol ester-inducible promoter.
  • Mouse CD69 is on chromosome 6 near NK gene complex; human CD69 is on chromosome 12p13 near NKG2 cluster.

Conclusions:

  • CD69 expression is tightly regulated upon T cell activation.
  • The CD69 gene's location suggests potential functional relationships with other immune genes.
  • The human CD69 gene's proximity to a region involved in childhood leukemia warrants further investigation.