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Changes in membrane potential associated with cell swelling and regulatory volume decrease in barnacle muscle cells

D M Berman¹, C Peña-Rasgado, H Rasgado-Flores

¹Department of Physiology and Biophysics, University of Health Sciences/Chicago Medical School, Illinois 60064.

The Journal of Experimental Zoology

|February 1, 1994

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Extracellular calcium (Cao) stabilizes membrane potential in barnacle muscle cells. Hypotonicity causes swelling, but Cao enables regulatory volume decrease (RVD) via a verapamil-sensitive calcium influx.

Area of Science:

Cellular physiology
Membrane biophysics

Background:

Cell volume regulation is crucial for cellular function.
Extracellular calcium and membrane potential are key factors influencing cell behavior.

Purpose of the Study:

To investigate the effects of hypotonicity and extracellular calcium (Cao) on barnacle muscle cell volume and membrane potential (VM).
To elucidate the role of Cao in regulatory volume decrease (RVD) and its interaction with cell swelling.

Main Methods:

Isolated barnacle muscle cells were subjected to hypotonic solutions with and without Cao.
Membrane potential (VM) and cell volume changes were monitored.
The effect of verapamil, a calcium channel blocker, on RVD was assessed.

Main Results:

Related Experiment Videos

Extracellular calcium (Cao) stabilized membrane potential (VM) and reduced depolarization rate.
Hypotonicity induced cell swelling, but RVD was observed only in the presence of Cao.
Verapamil inhibited Cao-dependent RVD, suggesting calcium influx is critical.
Cell swelling caused depolarization due to intracellular potassium ([K+]i) dilution.
RVD was associated with hyperpolarization, potentially due to increased [K+]i and pathway activation.

Conclusions:

Extracellular calcium (Cao) plays a vital role in stabilizing membrane potential (VM) in barnacle muscle cells.
Cell swelling triggers depolarization, explained by intracellular potassium ([K+]i) dilution.
A verapamil-sensitive calcium influx, activated by cell swelling, is essential for regulatory volume decrease (RVD).
Regulatory volume decrease (RVD) is accompanied by hyperpolarization, possibly linked to potassium dynamics and conductive pathway activation.