Vasoactive intestinal polypeptide precursor mRNA exhibits diurnal variation in the rat suprachiasmatic nuclei

¹Laboratory of Integrative Brain Function, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.

Brain Research. Molecular Brain Research

|November 1, 1993

Abstract:

Vasoactive intestinal polypeptide (VIP), which is synthesized in the ventrolateral subdivision of the suprachiasmatic nucleus (SCN), the circadian pacemaker in the mammalian brain, is suggested to mediate information on environmental light. Diurnal rhythms of VIP precursor mRNA in the SCN was determined in the present study by Northern blot hybridization with an antisense RNA probe in rats kept under light-dark conditions. VIP precursor mRNA was found most abundant during the dark phase, as light exposure suppressed VIP precursor mRNA. This suppression, however, did not last throughout the light phase and VIP precursor mRNA returned to the level before the onset of light after 8 h in light, suggesting that VIP mRNA responds to photic cues and not to light per se. This is in contrast to the sustained suppression of VIP peptide level in the SCN. It indicates that VIP mRNA is acting at a stage of light information processing upstream to VIP peptide in the circadian pacemaker.

Related Experiment Videos

Vasoactive intestinal polypeptide precursor mRNA exhibits diurnal variation in the rat suprachiasmatic nuclei

Related Concept Videos

Unique Genetic and Histological Signatures of Mouse Pericardial Adipose Tissue.

Defective NOTCH signaling drives increased vascular smooth muscle cell apoptosis and contractile differentiation in bicuspid aortic valve aortopathy: A review of the evidence and future directions.

The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice.

MNK1 and MNK2 mediate adverse effects of high-fat feeding in distinct ways.

Maternal high-fat diet and offspring expression levels of vitamin K-dependent proteins.

Effect of a low-protein diet during pregnancy on expression of genes involved in cardiac hypertrophy in fetal and adult mouse offspring.

Adenoviral interneuronal transportation after retrograde gene transfer in mice.

IGF-I protects cortical neurons against ceramide-induced apoptosis via activation of the PI-3K/Akt and ERK pathways; is this protection independent of CREB and Bcl-2?

Scutellariae radix extracts suppress ethanol-induced caspase-11 expression and cell death in N(2)a cells.

Two estrogen replacement therapies differentially regulate expression of estrogen receptors alpha and beta in the hippocampus and cortex of ovariectomized rat.

Molecular regulation of glutamate and GABA transporter proteins by clobazam during epileptogenesis in Fe(+++)-induced epileptic rats.

Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus.

A CCNA1 Missense Variant Associated With Chromatid Non-Disjunction in Abnormal-Headed Sperm and Male Infertility.

Genomic characterization and phylogenetic placement of Matryoshka RNA virus 1 associated with Plasmodium vivax malaria in Africa.

Venous Plexus of Batson and Its Important Role in the Development of Cryptococcal Meningitis.

The affective impact of mixed reality on medical students' learning outcomes in clinical skills: A preliminary study.

Reverse vaccinology and immunoinformatics identify multi-epitope vaccine candidates against molluscum contagiosum virus.

Assessing Demographic Characteristics, Microbiology, and Mortality Outcomes Associated With Vertebral Osteomyelitis: A National Single-Center Cohort Study in Malta.

Related Experiment Videos

Vasoactive intestinal polypeptide precursor mRNA exhibits diurnal variation in the rat suprachiasmatic nuclei

Abstract:

Related Concept Videos

Related Articles