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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Several mRNAs with variable 3' untranslated regions and different stability encode the human PR264/SC35 splicing

A Sureau1, B Perbal

  • 1Laboratoire d'Oncologie Virale et Moléculaire, Institut Curie, Orsay, France.

Proceedings of the National Academy of Sciences of the United States of America
|February 1, 1994
PubMed

Insights

The PR264/SC35 splicing factor is regulated by c-Myb in hematopoietic cells. Alternative splicing of 3' untranslated regions affects PR264/SC35 mRNA stability and expression levels.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Hematopoiesis

Background:

  • The PR264/SC35 splicing factor plays a crucial role in RNA splicing.
  • PR264/SC35 expression is linked to c-Myb in human hematopoietic cells.

Purpose of the Study:

  • To investigate the relationship between c-Myb and PR264/SC35 mRNA variants.
  • To determine how alternative 3' untranslated regions influence PR264/SC35 mRNA stability and expression.

Main Methods:

  • Analysis of PR264 mRNA variants and their 3' untranslated sequences.
  • Assessment of mRNA half-lives and correlation with 3' UTR length.
  • Investigation of c-Myb transactivation of PR264 mRNA expression.

Main Results:

  • Multiple PR264 mRNAs, transactivated by c-Myb, encode an identical PR264/SC35 polypeptide.
  • These mRNAs differ in their 3' untranslated regions (UTRs) and exhibit distinct half-lives.
  • A direct correlation exists between 3' UTR length and mRNA stability.

Conclusions:

  • Alternative splicing of 3' UTRs in PR264 mRNAs contributes to regulating PR264/SC35 protein levels.
  • This mechanism may be critical for controlling PR264/SC35 expression during hematopoietic differentiation.
  • PR264/SC35 concentration-dependent splicing activity suggests a feedback loop involving 3' UTRs.

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