Effect of cyclophosphamide on limb regeneration in stages of Bufo regularis reuss

Pre-amputation treatment in both stages (53 and 56) caused a profound decline in limb regeneration abilities and gave heteromorphic regenerates with smaller number of toes in the early stage, while in the later one, it suppressed completely the regeneration of toes or toe protuberances; otherwise partial or complete restoration of the shank. CY treatment concomitant with amputation caused intensive enhancement of regeneration in the early stage, while in the later one it activated regeneration to a lesser degree.