Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Making the connection: exploring classical concepts in normal and abnormal limb development using contemporary

U K Abbott1, J M Pisenti

  • 1Department of Avian Sciences, University of California, Davis 95616.

Progress in Clinical and Biological Research
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An avian model for comparative studies of insulin teratogenicity.

Anatomia, histologia, embryologia·1998
Same author

Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation.

Nature·1997
Same author

The chick limbless mutation causes abnormalities in limb bud dorsal-ventral patterning: implications for the mechanism of apical ridge formation.

Development (Cambridge, England)·1996
Same author

Shh, HoxD, Bmp-2, and Fgf-4 gene expression during development of the polydactylous talpid2, diplopodia1, and diplopodia4 mutant chick limb buds.

Developmental genetics·1996
Same author

Localization of the chicken PgK gene to chromosome 4p by fluorescence in situ hybridization.

The Journal of heredity·1994
Same author

Roles of adhesion molecules NCAM and tenascin in limb skeletogenesis: analysis with antibody perturbation, exogenous gene expression, talpid mutants and activin stimulation.

Progress in clinical and biological research·1993
Same journal

DNA homology and chromosome stability: a sensitive yeast genetic system for identifying double-stranded DNA damage.

Progress in clinical and biological research·2018
Same journal

Endotoxin and sepsis: molecular mechanisms of pathogenesis, host resistance, and therapy. Proceedings of the 4th Conference of the International Endotoxin Society. Nagoya, Japan, October 23-27, 1996.

Progress in clinical and biological research·1998
Same journal

The pathogenic role of LBP in gram-negative sepsis and septic shock.

Progress in clinical and biological research·1998
Same journal

The role of interleukin 6 in endotoxin-induced inflammatory responses.

Progress in clinical and biological research·1998
Same journal

Role of nitric oxide and reactive oxygen species in endotoxin shock.

Progress in clinical and biological research·1998
Same journal

Interaction of lipopolysaccharide with a mammalian lyso-phosphatidate acyltransferase (LPAAT) transfected into E. coli, and effect of lisofylline on LPAAT transfected into mammalian cells.

Progress in clinical and biological research·1998
See all related articles

Classical vertebrate limb development studies reveal conserved inductive signaling. This research explores gene-phenocopy pairings, like wingless-2 and retinoic acid, to understand limb patterning mechanisms.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Classical studies established inductive tissue interactions as crucial for vertebrate limb patterning.
  • These interactions are spatiotemporally restricted and conserved across species.
  • Limb development research utilizes experimental approaches like grafting, mutation analysis, and teratogenic treatments.

Purpose of the Study:

  • To investigate the molecular control of limb patterning mechanisms.
  • To explore gene-phenocopy pairings, specifically the wingless-2 mutation and retinoic acid treatment.
  • To examine the interaction between wingless-2 and eudiplopodia in abnormal pattern formation.

Main Methods:

  • Homologous and heterologous tissue grafting and recombination.

Related Experiment Videos

  • Analysis of limb mutations and their pleiotropic effects.
  • Phenocopying of developmental syndromes using chemical or physical treatments.

    • Main Results:

    • Identified conserved inductive signals in limb development.
    • Demonstrated that some developmental syndromes can be mimicked by teratogenic treatments.
    • Studied the specific gene-phenocopy pairing of wingless-2 mutation and retinoic acid-induced syndrome.

    Conclusions:

    • Inductive tissue interactions are fundamental to vertebrate limb patterning.
    • Gene mutations and environmental factors can disrupt limb development through conserved pathways.
    • Further research into gene-phenocopy relationships like wingless-2 provides insights into developmental mechanisms.