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Updated: Aug 12, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Published on: September 27, 2012
Melatonin receptors in purified cell nuclei of liver
D Acuña-Castroviejo1, M I Pablos, A Menendez-Pelaez
1Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284.
Abstract:
Specific binding of [125I]iodomelatonin to homogenates from purified rat liver nuclei was characterized. The binding is rapid, reversible, saturable and of high affinity. Specific binding seems to be found in the nuclear protein fraction, since after precipitation of the proteins with trichloroacetic acid, the specific binding disappeared. The Kd (180 +/- 20 pM) and Bmax (9.1 +/- 0.03 fmol/mg protein) values (mean +/- S.E.M.) agree with the melatonin concentration in nuclei and may imply a physiological locus for melatonin action.
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