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Green fluorescent protein as a marker for gene expression
M Chalfie1, Y Tu, G Euskirchen
1Department of Biological Sciences, Columbia University, New York, NY 10027.
Science (New York, N.Y.)
|February 11, 1994
Abstract:
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.