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Related Experiment Videos

Biological applications of atomic force microscopy

R Lal1, S A John

  • 1Department of Medicine, University of Chicago, Illinois 60637.

The American Journal of Physiology
|January 1, 1994
PubMed
Summary

Atomic Force Microscopy (AFM) offers high-resolution imaging and molecular force measurements of biological samples in their native environment. This powerful technique enables real-time monitoring of cellular processes and precise nanomanipulation of biomolecules.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) is a novel tool for visualizing the microworld of cells and biomolecules.
  • AFM provides three-dimensional imaging of biological specimens within a physiological environment.

Purpose of the Study:

  • To review the operating principles, accomplishments, and future potential of AFM in biological research.
  • To highlight AFM's capability for real-time monitoring of biochemical and physiological processes.
  • To showcase AFM's ability to measure molecular-level forces and perform nanomanipulation.

Main Methods:

  • High-resolution three-dimensional imaging of biological specimens.
  • Molecular force measurements during image acquisition.
  • In-situ monitoring of dynamic biological processes.

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  • Nanomanipulation of cellular structures and biomolecules.

    • Main Results:

    • AFM enables imaging of living cells, cytoskeleton, chromatin, plasmids, ion channels, and membranes.
    • Dynamic processes like fibrinogen polymerization and crystal growth have been studied.
    • Physicochemical properties such as elasticity and viscosity in living cells were measured.
    • Successful nanomanipulations include DNA dissection and transfer of synthetic structures.

    Conclusions:

    • AFM provides unprecedented insights into biological systems at the nanoscale.
    • The technique facilitates real-time observation and manipulation of cellular components.
    • AFM holds significant promise for advancing our understanding of molecular mechanisms and developing new biotechnologies.