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Related Experiment Videos

Temporal pattern of rat small intestinal gene expression with refeeding

R A Hodin1, J R Graham, S Meng

  • 1Department of Surgery, Beth Israel Hospital, Harvard Medical School, Boston, Massachusetts 02215.

The American Journal of Physiology
|January 1, 1994
PubMed
Summary

Refeeding adult rats after fasting induced rapid changes in small intestinal gene expression. Early responses involved immediate-early genes (c-fos, c-jun), followed by altered brush-border enzyme expression, revealing insights into intestinal growth and differentiation.

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Area of Science:

  • Molecular Biology
  • Gastroenterology
  • Cell Biology

Background:

  • Small intestinal epithelial growth is a complex process involving coordinated gene expression.
  • Understanding the molecular mechanisms of intestinal adaptation is crucial for addressing gastrointestinal disorders.

Purpose of the Study:

  • To elucidate the molecular mechanisms of small intestinal epithelial growth and adaptation.
  • To investigate the temporal patterns of gene expression in response to refeeding.

Main Methods:

  • Adult rats were fasted and then refed.
  • Histological examination assessed mucosal changes (atrophy and hyperplasia).
  • Northern blot analysis quantified gene expression alterations in small intestinal mucosal scrapings.

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Main Results:

  • Refeeding rapidly increased the expression of immediate-early genes c-fos and c-jun.
  • Later, gene expression for lactase decreased, while intestinal alkaline phosphatase (IAP) mRNA increased.
  • These changes reflect a return to the normal fed state phenotype.

Conclusions:

  • Demonstrated a complex, temporally regulated pattern of gene expression in the small intestine upon refeeding.
  • Provided insights into the relationship between intestinal growth and differentiation processes.
  • Highlighted the roles of immediate-early genes and brush-border enzymes in intestinal adaptation.