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Related Experiment Videos

Stretch preconditions canine myocardium

M Ovize1, R A Kloner, K Przyklenk

  • 1Heart Institute, Hospital of the Good Samaritan, Los Angeles 90017.

The American Journal of Physiology
|January 1, 1994
PubMed
Summary

Myocardial stretch, not just ischemia-reperfusion, can precondition the heart. This protective effect against heart damage is likely mediated by stretch-activated ion channels, as shown in canine studies.

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Area of Science:

  • Cardiology
  • Physiology

Background:

  • Ischemic preconditioning protects the heart from damage, typically triggered by brief ischemia-reperfusion.
  • Ischemia also causes transient heart dilation (stretch), suggesting stretch itself might be a preconditioning factor.

Purpose of the Study:

  • To investigate if myocardial stretch, induced by acute volume overload, can precondition the heart via stretch-activated ion channels.
  • To determine the role of stretch-activated ion channels in myocardial preconditioning.

Main Methods:

  • Forty-two anesthetized dogs were used. Interventions included acute volume overload, coronary artery occlusion, reperfusion, and Gd3+ (a stretch-activated channel blocker).
  • Dogs underwent either control, acute volume overload, or Gd3+ administration followed by various ischemic insults.

Main Results:

  • Myocardial stretch significantly reduced infarct size following a 60-minute ischemic insult.
  • Gd3+ completely blocked the protective effects of stretch and partially reversed the benefits of ischemic preconditioning.
  • Gd3+ alone did not alter the extent of necrosis.

Conclusions:

  • Myocardial stretch per se can precondition the canine heart, offering protection against ischemic injury.
  • Stretch-activated ion channels are likely involved in mediating the protective effects of myocardial stretch.
  • This finding expands the understanding of preconditioning mechanisms beyond traditional ischemia-reperfusion models.

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