Aging of nucleolar organizer region in rat basal forebrain neurons related to learning and memory

The nucleolar organizer of rat basal forebrain neurons was studied with histochemical and morphometrical techniques, in order to analyze quantitatively the morphological correlate of activity as it changes during learning and aging of the brain. The learning abilities of adult (2 months) and senile (30 months) rats were tested with a conditioned response learning paradigm. Four groups of rats were defined: one group consisted of untrained adults, a second group consisted of trained adults, and the senile rats were subdivided into one group, which was able to learn and another group which was not able to perform the test. Frozen sections through the basal nucleus were silver stained to visualize the nucleolar organizer and the area of its profile was measured. The data showed that the nucleolar organizer in both the trained adult and the senile rats who were able to learn, was much larger than that in the untrained adults and the senile rats who were not able to learn. In the latter groups the nucleolar organizers were of equal size. An additional finding was, that the senile rats who could not be trained, showed a lower neuronal density in the basal nucleus compared with that of the trainable senile and adult rats. The changes in the size of the nucleolar organizer are interpreted as a morphological sign of learning-induced increases in transcription and synthesis of ribosomal RNA.